Chun SO*, Garcia ET*, Rejas M*, Hayes ML. A Conserved Lysine in an Ion-Pair with a Catalytic Glutamate Is Critical for U-to-C RNA Editing but Restricts C-to-U RNA Editing. Biochemistry. 2025 Jan 7;64(1):15-19. doi: 10.1021/acs.biochem.4c00625. Epub 2024 Dec 9. PMID: 39653594; PMCID: PMC11713852.
Hayes ML, Garcia ET*, Chun SO*, Selke M. Crosslinking of base-modified RNAs by synthetic DYW-KP base editors implicates an enzymatic lysine as the nitrogen donor for U-to-C RNA editing. J Biol Chem. 2024 Aug;300(8):107454. doi: 10.1016/j.jbc.2024.107454. Epub 2024 Jun 7. PMID: 38852885; PMCID: PMC11332814.
Hua H. Y.*, Santibanez P. I.*, Ngo V. T.*, Hayes M. L. RIP-Seq analysis of non-PPR chloroplast editing factors reveals broad RNA interactions and enrichment of less efficiently translated RNAs by OZ1 and ORRM1 complexes. Plant J. 2024 Mar;117(5):1528-1542. doi: 10.1111/tpj.16581. Epub 2023 Dec 13. PMID: 38088241; PMCID: PMC10922338.
Boyd, R. D.*, Hayes, M. L., A ribonuclease activity linked to DYW1 in vitro is inhibited by RIP/MORF proteins. Sci Rep 2023, 13 (1), 10723.
Bentolila S., Gipson A. B., Kehl A. J., Hamm L. N., Hayes M. L., Mulligan R. M., Hanson M. R. A RanBP2-type zinc finger protein functions in intron splicing in Arabidopsis mitochondria and is involved in the biogenesis of respiratory complex I. Nucleic Acids Res. 2021 Apr 6;49(6):3490-3506. doi: 10.1093/nar/gkab066. PMID: 33660772; PMCID: PMC8034646.
Hayes, M. L., Santibanez, P. I.*, A plant pentatricopeptide repeat protein with a DYW-deaminase domain is sufficient for catalyzing C-to-U RNA editing in vitro. J Biol Chem 2020, 295 (11), 3497-3505.
Sandoval, R.*, Boyd, R. D.*, Kiszter, A. N.*, Mirzakhanyan, Y., Santibanez, P.*, Gershon, P. D., Hayes, M. L., Stable native RIP9 complexes associate with C-to-U RNA editing activity, PPRs, RIPs, OZ1, ORRM1 and ISE2. Plant J 2019, 99 (6), 1116-1126.
Diaz, M., Bentolila, S., Hayes, M. L., Hanson, M. R, Mulligan, R. M., A Protein with an Unusually Short PPR domain, MEF8, Affects Editing at over 60 Arabidopsis Mitochondrial C Targets of RNA Editing. Plant J 2017, 92:638-649 (doi: 10.1111/tpj.13709)
Hayes M. L., Dang K. N., Diaz M. F., Mulligan R. M. A conserved glutamate residue in the C-terminal deaminase domain of pentatricopeptide repeat proteins is required for RNA editing activity. J Biol Chem 2015, 290:10136-42. doi: 10.1074/jbc.M114.631630.
Hayes, M. L., Giang, K., Berhane, B. and Mulligan R. M. Identification of Two Pentatricopeptide Repeat Genes Required for RNA Editing and Zinc Binding by C-terminal Cytidine Deaminase-like Domains. J Biol Chem 2013, doi: 10.1074/jbc.M113.485755.
Hayes, M. L., Giang, K. and Mulligan R. M. Molecular evolution of pentatricopeptide repeat genes reveals truncation in species lacking an editing target and structural domains under distinct selective pressures. BMC Evolutionary Biology 2012, 12:66 (doi:10.1186/1471-2148-12-66).
Hayes, M. L., and Mulligan R. M. Pentatricopeptide repeat proteins constrain genome evolution in chloroplasts. Molecular Biology and Evolution 2011, 28:2029-2039 (doi:10.1093/molbev/msr023).
Heller, W. P., Hayes, M. L., and Hanson, M. R. Cross-competition in editing of chloroplast RNA transcripts in vitro implicates sharing of trans-factors between different C targets. J Biol Chem 2008, 283, 7314-7319.
Hayes, M. L., and Hanson, M.R. High conservation of a 5' element required for RNA editing of a C target in chloroplast psbE transcripts. J Mol Evol 2008, 67, 233-245.
Hayes, M. L., and Hanson, M.R. Identification of a sequence motif critical for editing of a tobacco chloroplast transcript. RNA 2007, 13, 281-288.
Hayes, M. L., and Hanson, M.R. Assay of editing of exogenous RNAs in chloroplast extracts of Arabidopsis, maize, pea, and tobacco. 2007. Methods Enzymol 424, 459-482.
Hayes M. L., Reed M. L., Hegeman C. E., and Hanson M. R. Sequence elements critical for efficient RNA editing of a tobacco chloroplast transcript in vivo and in vitro. Nucleic Acids Research. 2006 34: 3742-3754.
Hegeman C. E., Hayes M. L., Hanson M. R. Substrate and cofactor requirements for RNA editing of chloroplast transcripts in Arabidopsis in vitro. Plant J. 2005, 42(1):124-32. doi: 10.1111/j.1365-313X.2005.02360.x. PubMed PMID: 15773858.
*CSULA researchers mentored