Winter 2012 Biomedical Seminar Series
Friday, March 9, 2012
Using next-gen sequencing Â a genome-wide approach - to analyze transcription factor binding and gene expression during muscle development
Dr. Sandy Sharp
Department of Biological Sciences Center for Interdisciplinary Quantitative Analysis (CINQA)
ABSTRACT: Massively parallel Ânext-genÂ DNA sequencing has made it possible to determine where a transcription factor is bound throughout the entire mouse genome in a single experiment. Likewise, next-gen sequencing has made it possible to determine all of the RNAs being expressed in a single experiment. Analysis of where MyoD, a muscle-specific transcription factor, is bound throughout the genome in C2C12 pre-muscle cells, and correlation with gene expression has led to unexpected findings that challenge the scientific community to expand its current models for how MyoD works.