Poster Abstract

Winter 2012 Biomedical Seminar Series

Friday, March 2, 2012

Magneto-rheological fluid flow through micro-channels with the intension of isolating a polymer monolith

Velveth Klee
LSAMP VIII Bridge to the Doctorate Scholar
Irish Separation Science Cluster (ISSC), Dublin City University, Dublin, Ireland

This work investigates the capabilities of MRF to isolate polymers in silica capillaries. Polymer solution was manually pumped into capillaries followed by MRF. Air bubbles were created between the MRF and polymer solution to prevent mixing. A small neodymium magnet was used to move the MRF to the desired position to create barriers. Preliminary studies showing no polymerization occurring in the capillaries have been confirmed. The absence of polymerization is likely due to fluid interaction that changes the composition of the polymer solution.The resulting structure may be a new material that can perhaps be manipulated using heat and a magnetic field.

Measuring the Gene Expression Involved in Lipid Pathways of Bronchial Epithelial Cells in Cystic Fibrosis and in Response to Staphylococcus aureus Infections

Jessica Ponce
LSAMP VIII Bridge to the Doctorate Scholar
Summer Research Site: Department of Pediatrics, Allergy/Pulmonary, University of Iowa

Respiratory tract infections continue to be among the leading cause of illness and death, in particular in cystic fibrosis (CF), a genetic disease with mucus accumulating in the lungs. Bronchioepithelial cells (BECs) lining the internal surfaces of the respiratory tract play a role in innate immune defense by secreting antimicrobial peptides, cytokines and possibly antimicrobial lipids. In this study, microarray expression profiling was used to query changes in lipid pathways in pig BECs infected with CF and Staphylococcus aureus. Following reverse transcription of mRNA extracts microarrays were probed for gene sets involved in lipid pathways and computational analysis was performed to observe gene expression patterns involved in diseased compared to normal pigs. We found that various genes involved in lipid pathways were up-regulated in infected pigs. This study provides evidence that gene products involved in lipid pathways play a role in defense mechanisms to combat infection and may lead to novel anti-infectious therapy.

Centrifugally Driven Microextraction on a Microfluidic Disc

Judith Alvarado
Bridges to the PhD Fellow bIrish Separation Science Cluster, Biomedical Diagnostics
Institute National Center for Senor Research, Dublin City University, Dublin 9, Ireland

An eight channel centrifugal microfluidic disc for the microextraction of Vitamin B12 is described. The Solidworks program was used to design the dimensions of the microchannels. Zenor cyclic olefin copolymer (COC) and pressure sensitive adhesive films were used to create the centrifugal microfluidic disc. Monoliths were used in the disc as frits to hold 5µm silica beads. To quench the beads, water and acetonitrile were added before the vitamin solution. A 95µL solution of vitamin B12 was injected and extracted. UV/vis optical fibers were used as a detection method after extraction of the vitamin. Having successfully extracted the vitamin solution, the final step of the project involves comparing the spectra obtained via the UV/vis optical fibers to that using standard UV/vis spectroscopy. This work demonstrates the efficacy and potential of using centrifugally driven microextraction on a microfluidic platform. Further work is focused on utilizing this technology in more complex systems. References 1. Duffy, E. M.S. Dissertation, Dublin City University, 2011. 2. Vazquez, M.; Paull, B. Analytica Chimia Acta. 2010, 668, 100-113. 3. Moschou, E., et al. Anal Bioanal Chem. 2006, 385, 596-605. 4. Penrose, A.; Myers, P.; Bartle, K.; Mc Crossen, S. Analyst. 2004, 129, 704-709 5. Stachowaik, T. et al. Electrophoresis. 2003, 24, 3689-3693.

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