Off-campus Summer Research Presentations

January 7, 2005

Generation of AhR-tetracysteine Construct

Apollo Kacsinta

Abstract: Aryl hydrocarbon receptor (AhR or dioxin receptor) is a transcription factor that induces the production of xenobiotic metabolizing enzymes (XMEs).  The AhR usually resides in the cytosol with a couple of chaperone proteins, until it is bound by a ligand.  Once bound by a ligand, the AhR loses the chaperones and translocates to the nucleus where it eventually induces the production of XMEs.  We introduced a short sequence containing a tetracysteine (-Cys-Cys-Xaa-Xaa-Cys-Cys-) tag at the end of an AhR sequence.  We wanted to develop a labeling method that would allow us to determine if the AhR was activated and recent advances in labeling techniques using biarsenical dyes and tetracysteine motifs in Vivo showed promise.