Janurary 7, 2005

Optimizing a technique for generating recombinant adenoviruses

Maira Soto, Dr Luis P. Villarreal & Dr. Toai Nguyen,
Center for Virus Research,
UC Irvine

Abstract: For the past 17 years special enfaces has been placed on adenoviruses because of their ability to express foreign proteins and to transfer and express therapeutic genes. Furthermore, adenoviruses are advantageous to use for gene therapy because they can infect different cell types, both proliferating and quiescent, also their genomes do not integrate into the host cell genome. Although much research has been conducted on techniques for efficiently producing these vectors there are still aspects of their production that may be optimized. The rate-limiting step in the generation of recombinant adenoviruses is the cloning and so it is where the most work needs to be done. In our studies we will clone two different inserts, the two shorter fragments containing the green fluorescent protein gene (GFP) and the red fluorescent protein gene (RFP) as well as the longer lac Z insert. The inserts we are using were chosen because both segments are easy markers to test the production of the recombinant adenoviruses. In all cases the aim of the study is to optimize the technique for future experiments.