April 16, 2010
The Potential of Lipid-Based Antibiotics for the Treatment of Cancer
Cancer Collaborative Scholar
The increasing resistance of cancer cells to conventional chemotherapeutics has raised the need for alternative treatments. Previously, antibiotics have been used as anticancer agents. We have recently discovered that selected host-derived lipids exhibit antimicrobial activity. We hypothesize that some of these may also exert anticancer effects and may be developed into novel anticancer agents. Eleven different cholesteryl esters and free fatty acids were packaged into two liposomal formulations designated C and D and tested against the breast cancer cell line MCF-7 and the liver carcinoma cell line HepG2 employing a metabolic assay (XTT) for screening and a protein based assay (SRB) to determine the IC50. Among the lipids tested cholesteryl oleate and linoleic acid effected greater than 90% metabolic inhibition of MCF7 cells, and IC50 of 64 and 16 ?g/mL, respectively, was established. These data suggest that host derived lipids may represent a novel pipeline of anticancer agents. References Lu, I. et al. 2009. Docosahexaenoic acid induces proteasome-dependent degradation of estrogen receptor alpha and inhibits the downstream signaling target in MCF-7 breast cancer cells. Journal of Nutritional Biochemistry. doi:10.1016/j.jnutbio.2009.02.009 Do, T. et al. 2008. Lipids Including Cholesteryl Linoleate and Cholesteryl Arachidonate Contribute to the Inherent Antibacterial Activity of Human Nasal Fluid. Journal of Immunology. 181(6):4177-87.