Friday, April 18, 2008

Does the Mutation Found in the P Protein of Sendai Virus Variant, F1-R, Contribute to Decreased Viral Production Rate?
Shandee Dixon, MBRS-RISE MS to PhD Scholar

F1-R, a pantropic variant of Sendai virus, consistently produces fewer viral particles that wild-type virus. We originally attributed this to the amino acid substitutions in the M protein. However, studies with reverse genetics viruses generated to identify the amino acid substitutions necessary for the pantropism of F1-R proved that amino acid substitutions in F1-R M are not responsible for F1-R’s reduced rate of virus production.  F1-R has mutations in both the P and L genes that encode the viral polymerase complex proteins. This study was initiated to test the hypothesis that the F1-R P gene mutation at position 2307 is responsible for F1-R’s reduced rate of virus production. To test this hypothesis, the P2307 mutation was introduced into pRGV0, a plasmid containing the cDNA of Sendai virus.  Reverse genetics will be used to recover the mutant virus and the virus production rate of the recovered virus will be studied.