PowerPoint Presentation - Differential regulation of Id1, Id2, andTwist in myogenesis and osteogenesis

Id-1 andTwist-1 Regulation in MSC-hTERT line
oTo compare Id-1 and Twist-1 regulation
Grow cells inmaintenance medium
Maintaincells at 70%-80%confluency
Obtain mRNAfrom cells at varioustime points
Change mediumevery 3 days
Introduceosteogenic medium topromote differentiation
PerformQuantitative PCR 
Analyze Id-1and Twist-1expression