oCells were grown in expansion medium
oAlpha-Minimal Essential Medium supplemented with Fetal bovine serum, penicillin, streptomycin, L-glutamine,and ascorbic acid 2- phosphate.
oDifferentiation was induced by changing medium conditions.
oExpansion medium was supplemented with dexamethasone, sodium pyruvate, hepes, and inorganic phosphate to induce differentiation to bone.
oDifferentiation was carried out for 28 days
oRNA was collected at days 2, 4, 7, 14, 21, and 28
oExpression of the genes listed above along with bone marker genes was measured by real time RT PCR