PowerPoint Presentation - Differential regulation of Id1, Id2, andTwist in myogenesis and osteogenesis

Id-1and Twist-1 Regulation in MSC-hTERT line
oTocompare Id-1 and Twist-1 regulation
Grow cells in maintenance medium
Maintain cells at 70%-80% confluency
Obtain mRNA from cells at various time points
Change medium every 3 days
Introduce osteogenic medium to promote differentiation
Perform Quantitative PCR 
Analyze Id-1 and Twist-1 expression