PowerPoint Presentation - Cancer Cell Culturing and CytotoxicityAssays for Anticancer Screening at City of Hope

The sulforhodamine B (SRB) Assay
nSRB binds to proteincomponents of cell
nSRB is bright-pinkaminoxanthene dye and binds basic amino groups– binding is stoichiometric to cell mass
nAdaptable to 96-well, high throughput screening
nDose ResponseAnalysis
Vichai,V., Kirtikara, K. (2006). Sulforhadamine B colorimetric assay for cytotoxicityscreening. Nature Protocols 1: 1112-1116.